Dr. Durg Vijai Singh

We have successfully shown the clonal relationships between clinical and environmental isolates of V. cholerae, indicating aquatic environment serve as a reservoir and transmission of the disease cholera. Our group is the first to report the occurrence of SXT ICE, dfr18, sulII, strAB, and aadA5 genes in environmental V. cholerae O1, and non-O1, and non-O139 strains isolated before 1992 from Varanasi, India, and showed acquisition of SXT ICEs and antibiotic resistance genes from other bacteria. Also, we have developed several multiplex PCRs for rapid detection of V. cholerae serogroups, virulence genes, SXT and antibiotic resistance genes, cadmium/zinc, and antiseptic resistance in PVL-positive and -negative S. aureus and CoNS. An MLST database for S. haemolyticus and Vibrio cholerae was developed and made available on the PubMLST.org website. Multidrug-resistant S. aureus and S. haemolyticus, including untypeable strains producing biofilm and matrix belonging to eDNA and/or protein, was also reported. We deciphered the contribution of eDNA in V. cholerae biofilm integrity and its interaction with VPS that provides strength to the biofilm. We provided evidence that phytomolecules: quercetin and naringenin modulate the quorum-sensing pathway rather than killing the bacteria and can be used as an anti-quorum-sensing molecule for therapy against the pathogen.

1. Singh, D.V., & Sanyal, S,C. (1992). Haemolysin and enterotoxin production by Aeromonas caviae isolated from diarrhoeal patients, fish and environment. Journal of Diarrhoeal Diseases Research, 10, 16–20.
2. Singh, D.V., & Sanyal, S.C. (1992). Enterotoxicity of clinical and environmental isolates of Aeromonas spp. Journal of Medical Microbiology, 36, 269–272.
3. Singh, D.V., & Sanyal, S.C. (1992). Production of haemolysin and its correlation with enterotoxicity in Aeromonas spp. Journal of Medical Microbiology, 37, 262-267.
4. Singh, D.V., & Sanyal, S.C. (1992). Production of chitinase by enterotoxigenic Aeromonas species isolated from clinical and environmental sources. Journal of Diarrhoeal Diseases Research, 10, 213–216.
5. Singh, D.V., & Sanyal, S.C. (1992). Biochemical characteristics and enterotoxicity of Aeromonas species isolated from man and environment. Journal of Diarrhoeal Diseases Research, 10, 231-234.
6. Singh, D.V., & Sanyal, S.C. (1993). Haemagglutinating activity, serum sensitivity and enterotoxicity of Aeromonas spp. Journal of Medical Microbiology, 38, 49–53.
7. Singh, D.V., Dubey, R.S., & Sanyal, S.C. (1993). Adherence of haemagglutinating and non-haemagglutinating clinical and environmental isolates of Aeromonas. Journal of Diarrhoeal Diseases Research, 11, 157-160.
8. Singh, D.V., & Sanyal, S.C. (1994). Antibiotic resistance in clinical and environmental isolates of Aeromonas spp. Journal of Antimicrobial Chemotherapy,  33, 368–369.
9. Tikoo, A., & Singh, D.V. (1994). Sanyal SC. Influence of animal passage on haemolysin and enterotoxin production in Vibrio cholerae O1 biotype El Tor stains. Journal of Medical Microbiology, 40, 246–251.
10. Singh, D.V., & Sanyal, S.C. (1995). Relationship between enterotoxicity and multiple drug resistance in Aeromonas spp. Journal of Diarrhoeal Diseases Research, 13, 172–175.
11. Shukla, B.N., Singh, D.V., & Sanyal, S.C. (1995). Attachment of non-culturable toxigenic Vibrio cholerae O1 and non-O1 and Aeromonas spp. to the aquatic arthropod Gerris spinolae and plants in the River Ganga, Varanasi. FEMS Immunology and Medical Microbiology, 12, 113–120.
12. Singh, D.V., Tikoo, A., & Sanyal, S.C. (1996).  Production of the new cholera toxin by environmental isolates of Vibrio cholerae non-O1. Journal of Medical Microbiology, 45, 31–34.
13. Singh, D.V., Tikoo, A., & Sanyal, S.C. (1996). Haemolysin produced by Vibrio cholerae non-O1 is not enterotoxic. Journal of Medical Microbiology, 45, 35–39.
14. Tikoo, A., Singh, D.V., Shukla, B.N., & Sanyal, S.C. (1996). Development of an improved synthetic medium for a better production of the new cholera toxin and its immunological relationship with the toxin produced by Vibrio cholerae O139 strains. FEMS Immunology and Medical Microbiology, 14, 67–72.
15. Singh, D.V., & Sanyal, S.C. (1996). Production of haemolysin and enterotoxin by Aeromonas jandaei and Aeromonas trota after animal passage. Journal of Diarrhoeal Diseases Research, 14, 274–279.
16. Singh, D.V., Tikoo, A., & Sanyal, S.C. (1996). Candidate live oral cholera vaccine strains produce a new cholera toxin. Current Science, 70, 237–238.
17. Singh, D.V., Tikoo, A., & Sanyal, S.C. (1996). Cholera toxin zonula occludens toxin and accessory cholera enterotoxin gene negative Vibrio cholerae non-O1 strains produce the new cholera toxin. Current Science, 70, 1041–1043.
18. Singh, D.V., & Sanyal, S.C. (1997). Enterotoxicity, haemolytic activity and antibiotic susceptibility of Aeromonas eucrenophila strains isolated from water and infected fish. Indian Journal of Experimental Biology, 35,144–147.
19. Singh, D.V., & Sanyal, S.C. (1997). Enteropathogenicity of Aeromonas jandaei and A. trota. FEMS Immunology and Medical Microbiology, 17, 243–250.
20. Singh, D.V., & Sanyal, S.C. (1999).Virulence patterns of Aeromonas eucrenophila isolated from water and infected fish. Journal of Diarrhoeal Diseases Research, 17, 37-42.
21. Singh, D.V. (1999). Virulence factors in Aeromonas veronii bv veronii. Journal of Diarrhoeal Diseases Research, 17, 90-91.
22. Singh, D.V. (2000). A putative heat-labile enterotoxin expressed by strains of Aeromonas media. Journal of Medical Microbiology, 49, 685-689.
23. Singh, D.V. (2000). Production of heat labile enterotoxin by strains of Aeromonas veronii bv veronii. Journal of Health Population and Nutrition, 18, 44-48.
24. Singh, D.V., Matte, M.H., Matte, G.R., Ziang, S., Sabeena, F., Shukla, B.N., Sanyal, S.C., Huq, A., & Colwell, R.R. (2001). Molecular analysis of Vibrio cholerae O1, O139, non-O1, and non-O139 strains: clonal relationships between clinical and environmental isolates. Applied and Environmental Microbiology,  67(2), 910-921. (IF: 3.688)
25. Sabeena, F., Thirivikramji, G., Radhakutty, G., Indu, P., & Singh, D.V. (2001). In vitro susceptibility of Vibrio cholerae O1 biotype El Tor strains associated with an outbreak of cholera in Kerala, Southern India. Journal of Antimicrobial Chemotherapy, 47, 361-362. (IF: 3.490)
26. Singh, D.V., Isac, S.R., & Colwell, R.R. (2002). Development of a hexaplex PCR assay for rapid detection of virulence and regulatory genes in Vibrio cholerae and Vibrio mimicus. Journal of Clinical Microbiology, 40(11), 4321-4324. (IF: 3.565)
27. Bhanumathi, R., Sabeena, F., Isac, S.R., Radhakutty, G., & Singh, D.V. (2002). Characterization of a toxigenic Vibrio cholerae O139 strain belonging to a new ribotype and isolated from a diarrheal patient. Journal of Clinical Microbiology, 40(12), 4779-4781. (IF: 3.565)
28. Bhanumathi, R., Isac, S.R., Shukla, B.N., & Singh, D.V. (2003). Molecular characterization of Vibrio cholerae O139 Bengal isolated from water and the aquatic plant Eichhornia crassipes in the River Ganga, Varanasi, India. Applied and Environmental Microbiology, 69(4), 2389-2394. (IF: 3.820)
29. Singh, D.V., Bhanumathi, R., & Colwell, R.R. (2004). Characterization of a cholera toxin-gene negative clinical strain of Vibrio cholerae O139. Journal of Clinical Microbiology,  42, 1381. (3.439)
30. Mantri, C.K., Mohapatra, S.S., Ramamurthy, T., Ghosh, R., Colwell, R.R., & Singh, D.V. (2006). Septaplex PCR assay for rapid identification of Vibrio cholerae including detection of virulence and intSXT genes. FEMS Microbiology Letters, 265(2), 208-214. (IF: 2.068)
31. Mohapatra, S.S., Ramachandran, D., Mantri, C.K., & Singh, D.V. (2007). Characterization of the genetic background of Vibrio cholerae O1 biotype El Tor serotype Inaba strains isolated in Trivandrum, Southern India. Journal of Medical Microbiology, 56(2), 260-265. (IF: 2.091)
32. Ramachandran, D., Bhanumathi, R., & Singh, D.V. (2007). Multiplex PCR for detection of antibiotic resistance genes and the SXT element: application in the characterize Vibrio cholerae. Journal of Medical Microbiology, 56(3), 246-251. (IF: 2.091)
33. Patra, M., Pandey, S.K., Ramamurthy, T., Singh, D.V., & Das, S.K. (2007).  Characterization of cytotoxin producing Aeromonas caviae (strain HT10) isolated from a sulfur spring in Orissa, India. Letters in Applied Microbiology, 44, 338-341. (IF:  1.623)
34. Mohanty, A., Kar, P., Mishra, K., Singh, D.V., Mohapatra, N., Kar, S.K., Dash, A.P., & Hazra, R.K. (2007). Multiplex PCR assay for the detection of Anopheles fluviatilis species complex, human host preference, and Plasmodium falciparum sporozoite presence, using a unique mosquito processing method. American Journal of Tropical Medicine & Hygiene, 76(5), 837-843. (IF: 2.183)
35. Thomas, S., Ramachandran, D., Remani, B., & Singh, D.V. (2008). Detection and molecular characterization of Vibrio cholerae O1 Inaba biotype El Tor strain in Kerala, S India. World Journal of Microbiology and Biotechnology, 24, 433-434. (IF: 0.945)
36. Mohapatra, H., Mohapatra, S.S., Mantri, C.K., Colwell, R.R., & Singh, D.V. (2008). Vibrio cholerae non-O1, non-O139 strains isolated before 1992 from Varanasi, India are multiple drug resistant, contain intSXT, dfr18 and aadA5 genes. Environmental Microbiology, 10(4), 866-873. (IF: 4.707)
37. Mohapatra, S.S., Ramachandran, D., Mantri, C.K., Colwell, R.R., & Singh, D.V. (2009). Determination of relationships among non-toxigenic Vibrio cholerae O1 biotype El Tor strains from housekeeping gene sequences and ribotype patterns. Research in Microbiology, 160, 57-62. (IF: 2.154)
38. Mohanty, A., Swain, S., Singh, D.V., Mahapatra, N., Kar, S.K., & Hazra, R.K. (2009). A unique methodology for detecting the spread of chloroquine-resistant strains of Plasmodium falciparum, in previously unreported area, by analyzing anophelines of malaria endemic zones of Orissa, India. Infection, Genetics and Evolution, 9(4), 462-467. (IF: 3.223)
39. Mohapatra, S.S., Mantri, C.K., Mohapatra, H., Colwell, R.R., & Singh, D.V. (2010). Analysis of clonally related environmental Vibrio cholerae O1 El Tor isolated before 1992 from Varanasi, India reveals origin of SXT-ICEs belonging to O139 and O1 serogroups. Environmental Microbiology Reports, 2(1), 50-57. (IF: 3.076)
40. Mantri, C.K., Mohapatra, S.S., Colwell, R.R., & Singh, D.V. (2010). Sequence analysis of Vibrio cholerae orfU and zot from pre-CTXΦ and CTXΦ reveals multiple origin of pre-CTXΦ and CTXΦ. Environmental Microbiology Reports,  2(1), 67-75. (IF: 3.076)
41. Turabe Fazil, M.H.U., Kumar, S., Subbarao, N., Pandey, H.P., & Singh, D.V.  Homology modeling of a sensor histidine kinase from Aeromonas hydrophila, Journal of Molecular Modeling, 16(5), 1003-1009. (IF: 1.871)
42. Mantri, C.K., Mohapatra, S.S., & Singh, D.V. (2010). Effect of storage and sodium chloride on excision of CTXФ or pre-CTXФ and CTXФ from Vibrio cholerae O139 strains. Infection, Genetics and Evolution, 10, 925-930. (IF: 3.086)
43. Turabe Fazil, M.H.U., Bhanumathi, R.,, Pandey, H.P., & Singh, D.V. (2011). Characterization of Vibrio cholerae O139 belonging to multiple ribotypes and isolated from diarrhoeal patients in Kerala, southern India.  Infection, Genetics and Evolution, 11, 454-459. (IF: 3.128)
44. Mohapatra, S.S., Mantri, C.K., Fazil, M.H.U.T., & Singh, D.V. (2011). Vibrio cholerae O1 biotype El Tor strains isolated in 1992 from Varanasi, India harbored El Tor CTXФ and classical ctxB on the chromosome-I and classical CTXФ and classical ctxB on the chromosome-II. Environmental Microbiology Reports, 3(6),  783-790. (IF: 3.232)
45. Turabe Fazil, M.H.U., Kumar, S., Former, R., Pandey, H.P., & Singh, D.V. (2012).  Binding efficiencies of carbohydrate ligands with different genotypes of cholera toxin B: molecular modeling, dynamics and docking simulation studies. Journal of Molecular Modeling, 18(1), 1-10. (IF: 1.797-2011)
46. Isac, S.R., Nair, G.B., & Singh, D.V. (2012). Purification and characterization of cytotoxin produced by a clinical isolate of Vibrio cholerae O54 TV113. Applied Biochemistry and Biotechnology, 167(4), 809-823. (IF:1.943-2011)
47. Fazil, M.H.U.T., Kumar, S., Subba Rao, N., Selvaraj, C., Singh, S.K., Pandey, H.P., & Singh, D.V. Comparative structure analysis of two proteins belonging to quorum sensing system in Vibrio cholerae. Journal of Bimolecular Structure and Design,  30(5), 574-584. (IF: 4.986-2010)
48. Soni, D.K., Singh, R.K., Singh, D.V., & Dubey, S.K. (2013). Characterization of Listeria monocytogenes isolated from water of the River Ganga, human and milk in Varanasi, India. Infection, Genetics and Evolution 14, 83-91. DOI: 10.1016/j.meegid.2012.09.019 (IF: 3.128)
49. Kar, S., Panda, S., Sharma, S., Singh, D.V., Das, S., Sahu, S.K., & Das, T. (2013).  Comparison of type of species and antibacterial susceptibility profile of staphylococci isolated from normal healthy conjunctiva and ocular infections. Asia-Pacific Journal of Ophthalmology, 2(6), 365-371. (OnlineFirst 26.03.2013).
50. Panda, S., Kar, S., Choudhury, R., Sharma, S., & Singh, D.V. (2014). Development and evaluation of hexaplex PCR for rapid detection of methicillin, cadmium/ zinc and antiseptic resistant staphylococci, with simultaneous identification of PVL-positive and- negative Staphylococcus aureus and coagulase negative staphylococci. FEMS Microbiology Letters, 352, 114-122. (IF: 2.046)
51. Soni, D., Singh, M., Singh, D.V., & Dubey, S.K. (2014). Virulence and genotypic characterization of Listeria monocytogenes isolated from vegetable and soil samples. BMC Microbiology, 14, 241. DOI: 10.1186/s12866-014-0241-3 (IF: 2.98)
52. Mohapatra, S.S., Mantri, C.K., Bhotra, T., & Singh, D.V. (2015). Characteristics of Vibrio cholerae O1 isolated from water of the River Ganga, Varanasi, India. Indian Journal of Medical Microbiology, 33, 507-515. (IF: 1.037)
53. Soni, D., Singh, D.V., & Dubey, S.K. (2015). Pregnancy-Associated listeriosis: virulence and genotypic analysis of Listeria monocytogenes from clinical samples. Journal of Microbiology, 53(9), 653-60. (IF: 1.621)
54. Panda, S., & Singh, D.V. (2016). Whole genome sequences of Staphylococcus haemolyticus isolated from infected eye and healthy conjunctiva in Bhubaneswar, India. Genome Announcement, 4(2): e00099-16.
55. Bhotra, T., & Singh, D.V. (2016). Whole genome sequence of Vibrio alginolyticus isolated from the mucus of the Coral Fungia danai in the Andaman Sea, India. Genome Announcement, 4(3), e00339-16.
56. Panda, S., Kar, S., Sharma, S., & Singh, D.V. (2016). Multidrug-resistant Staphylococcus haemolyticus isolates from infected eye and healthy conjunctivae. Journal of Global Antibiotic Resistance, 6(3), 154-159. (IF: 1.276)
57. Das M.M., Bhotra, T. Zala, D., & Singh, D.V. (2016). Phenotypic and genetic characteristics of Vibrio cholerae O1 carrying Haitian ctxB and attributes of classical and El Tor biotypes isolated from Silvassa, India. Journal of Medical Microbiology, 65(8), 720-728. (IF: 2.159)
58. Panda, S., Jena, S., Sharma, S., Dhawan, B., Nath, G., & Singh, D.V. (2016). Identification of novel sequence types among Staphylococcus haemolyticus isolated from variety of infections in India. PLoS One, 11(11), e0166193. (IF: 3.54)
59. Bhotra, T., Das, M.M., Pal, B.B., & Singh, D.V. (2016). Genomic profile of antibiotic resistant, classical ctxB positive Vibrio cholerae O1 biotype El Tor isolated in 2003 and 2005 from Puri, India: a retrospective study. Indian Journal of Medical Microbiology, 34(4), 462-470. (IF: 1.149)
60. Jha, G., Sahu, P.K., Panda, S., Singh, D.V., Patole, S., Mohapatra, H., & Sarkar, M. (2017). Synthesis, phytophysical studies on some anthracene based ionic liquids and their application as biofilm inhibitors. Chemistry Select, 2(8), 2426-2432. DOI:10.1002/slct.201601964
61. Jena, S., Panda, S., Nayak, K., & Singh, D.V. (2017). Identification of major sequence types among multidrug-resistant Staphylococcus epidermidis strains isolated from infected eyes and healthy conjunctiva. Frontiers in Microbiology, 8, 1430. doi:10.3389/fmicb.2017.01430 (IF: 4.076)
62. Angermeyer, A., Das, M.M., Singh, D.V., & Seed, K.D. (2018). Analysis of 19 highly conserved Vibrio cholerae bacteriophages isolated from environmental and patient sources over a twelve-year period. Viruses, 10(7), 299; doi:10.3390/v10060299 (IF: 3.465)
63. Panda, S., & Singh, D.V. (2018). Biofilm formation by ica-negative ocular isolates of Staphylococcus haemolyticus. Frontiers in Microbiology, 9, 2687. doi:10.3389/fmicb.2018.02687. (IF: 4.076)
64. Aggrawal, S., Jena, S., Panda, S., Sharma, S., Dhawan, B., Nath, G., Singh, N.P., Nayak, K.C., & Singh, D.V. (2019). Antibiotic susceptibility, virulence pattern, and typing of Staphylococcus aureus strains isolated from variety of infections in India. Frontiers in Microbiology, 10, 2763. doi: 10.3389/fmicb.2019.02763 (IF: 4.285)
65. Mishra, M., Barik, S., Panda, S., Sarkar, A., Singh, D.V., & Mohapatra, H. (2020). Antibiotic resistance profile, outer membrane proteins, virulence factors, and genome sequence analysis reveal clinical isolates of Enterobacter are potential pathogens compared to environmental isolates. Frontiers in Cellular and Infection Microbiology, 10, 54. doi: 10.3389/fcimb.2020.00054. (IF: 4.3)
66. Aggarwal, S., & Singh, D.V. (2020). Efflux pumps and biofilm formation by MRSA and MSSA isolates of Staphylococcus aureus. Indian Journal of Experimental Biology, 58(8), 527-538. (IF: 1.050)
67. Kanampalliwar, A., & Singh, D.V. (2020). Virulence pattern and genomic diversity of Vibrio cholerae O1 and O139 strains isolated from clinical and environmental sources in India. Frontiers in Microbiology, 11, 1838. doi: 10.3389/fmicb.2020.01838. (IF: 4.285)
68. Kanampalliwar, A., & Singh, D.V. (2020). Extracellular DNA builds and interacts with vibrio polysaccharide in the biofilm matrix formed by Vibrio cholerae. Environmental Microbiology Reports, 12(5), 594-606. doi:10.1111/1758-2229.12870. (IF: 2.650)
69. Alam,, Jawed., Dilnawaz, F., Sahoo, S.K., Singh, D.V., Mukhopadhyay, A.K., Hussain, Tahziba., & Pati, S. (2022). Curcumin encapsulated into biocompatible co-polymer PLGA nanoparticle enhanced anti-gastric cancer and anti-Helicobacter pylori effect. Asian Pacific Journal of Cancer Prevention, 23(1):61-70. (IF: 1.77)
70. Bose, Swagata., Singh, D.V., Adhiya, A.K., & Acharya, N. (2023). Escherichia coli, but not staphylococcus aureus, functions as a chelating agent that exhibits antifungal activity against the pathogenic yeast Candida albicans. Journal of Fungi, 9: 286. https://doi.org/10.3390/jof9030286 (IF: 5.74)
71. Saha, Subhasree., Aggarwal, Shifu., & Singh, D.V. (2023). Attenuation of quorum sending system and virulence in Vibrio cholerae by phytomolecules. Frontiers in Microbiology, 10: doi: 10.3389/fmicb.2023.1133569 (IF: 6.06)
72. Das, M.M., & Singh, D.V. (2023). Microarray and whole-genome sequence analysis of CTX+ strain VCho146P and CTX- strain VCho146N of Vibrio cholerae O139 in relation to virulence and biofilm formation. in press.

• • 1. Singh, D.V. (2003). Hexaplex PCR for rapid detection of virulence factors. Expert Review in Molecular Diagnostics, 361, 781-784. (IF: 2.425)
    2. Singh, D.V., & Mohapatra, H. (2008). Application of DNA based methods in typing Vibrio cholerae strains. Future Microbiology, 3(1), 87-96. (IF: 1.976)
    3. Fazil, M.H.U.T., & Singh, D.V. (2011). Vibrio cholerae infection, novel drug targets and phage therapy. Future Microbiology, 6(10), 1199-1208. (IF: 3.819)
    4. Choudhury, R., Panda, S., & Singh, D.V. (2012). Emergence and dissemination of antibiotic resistance: a global problem. Indian Journal of Medical Microbiology, 30(4), 384-390. (IF: 0.988)
    5. Bose, S., Aggarwal, S., Singh, D.V., & Acharya, N. (2020). Extracellular vesicle: An unexplored weapon of gram-positive bacteria. Microbial Cell, 7(12), 312-322.

• • 1. 73. Singh, D.V. (2003). Hexaplex PCR for rapid detection of virulence factors. Expert Review in Molecular Diagnostics, 361, 781-784. (IF: 2.425)
    2. Singh, D.V., & Mohapatra, H. (2008). Application of DNA based methods in typing Vibrio cholerae strains. Future Microbiology, 3(1), 87-96. (IF: 1.976)
    3. Fazil, M.H.U.T., & Singh, D.V. (2011). Vibrio cholerae infection, novel drug targets and phage therapy.  Future Microbiology, 6(10), 1199-1208. (IF: 3.819)
    4. Choudhury, R., Panda, S., & Singh, D.V. (2012). Emergence and dissemination of antibiotic resistance: a global problem. Indian Journal of Medical Microbiology, 30(4), 384-390. (IF: 0.988)
    5. Bose,  S., Aggarwal, S., Singh, D.V., & Acharya, N. (2020). Extracellular vesicle: An unexplored weapon of gram-positive bacteria. Microbial Cell, 7(12), 312-322.

• 1. Singh, D.V., & Sanyal, S.C. (1992). Virulence factors of Aeromonas spp. In: P. Gopalakrishnakone & C.K. Tan (Eds.), Recent Advances in Toxinology, (pp. 291-307). National University of Singapore.
  2. Thomas, S., Praveen Kumar, P.P., Wilson, P.A., Bibin, T., & Singh, D.V. Molecular monitoring of Vibrio cholerae in the aquatic environment of Kerala. In: P. Natarajan, C.S. Paulose, I.S. Bright Singh, & K. Pillai (Eds.), Environment and Health, (pp. 67-78). Rajiv Gandhi Chair in Contemporary Studies, Cochin University of Science and Technology, Cochin.

1. Singh, D.V. (2005) Vibrio cholerae. In: J. Fuch & M. Podda (Eds.), Encyclopedia of Medical Genomics and Proteomics (pp. 1307-1311). Informa Healthcare.
2. 78. Mohapatra, H., Mohapatra, S.S., Mantri, C.K. & Singh, D.V. (2008). Horizontal gene transfer and ICEs: a perspective with reference to SXT ICEs in Vibrio cholerae. In: A. Pandey, C. Larroche, C.R. Soccol & C-G. Dussap (Eds.), New Horizon in Biotechnology (pp. 388-403). Asiatech Publishers, Inc, New Delhi.
3. Fazil, M.H.U.T., Kumar. S. & Singh, D.V. (2011). In silico analysis of sequence variations in cholera toxin B subunit and their binding with carbohydrate ligands. In: H.K. Dhingra, P.N. Jha & P. Bajpai (Eds.), Current Topics in Biotechnology and Microbiology, (pp. 168-177). LAP Lambert Academic Publishing AG & Co. KG, Dudweller Landstr, Germany. (ISBN 978-3-8443-2975-9)
4. Choudhury, R., Panda, S., Sharma, S. & Singh, D.V. (2012). Staphylococcal infection, antibiotic resistance and therapeutics. Chapter 10, In: Maria Pana (Ed.), Antibiotic Resistant Bacteria - A Continuous Challenge in the New Millennium, (pp. 247-272). InTech Publication, Croatia. ISBN: 978-953-51-0472-8, InTech, Available from: http://www.intechopen.com/books/antibiotic-resistant-bacteria-a-continuous-challenge-in-the-new-millennium/staphylococcal-infection-antibiotic-resistance-and-therapeutics
5. Singh, D.V. & Bhotra, T. (2014). 240-Cholera Disease. In: Michael Caplan (Ed.), Reference Module in Biomedical Sciences, (pp. 1-12). Elsevier Inc, San Diego, USA. ISBN: 978-0-12-801238-3 http://dx.doi.org/10.1016/B978-0-12-801238-3.00142-2
6. Saha, S. & Singh, D.V. (2020). Mechanism of antibiotic resistance and pathogenicity of Vibrio cholerae. Chapter 16, In: B. Siddhartha, M. Dyavaiah & A. Syed (Eds.), Model Organisms for Microbial Pathogenesis, Biofilm Formation, and Antimicrobial Drug Discovery, (pp. 273-299). Springer, Singapore. ISBN: 978-981-15-1694-8 https://doi.org/10.1007/978-981-15-1695-5_15

• Awards :
1. ASM Indo-US Visiting Research Professorship (2008)
2. GBF International Training Fellowship (2002)
3. Visiting Fellowship of UMSM, Baltimore, USA (1998)
4. UNESCO Short – Term Training Fellowship in Biotechnology (1998)
5. AMI Best poster award (1998)
• Projects:
1. A diagnostic panel for rapid detection of early-onset sepsis in high-risk neonates. DBT-ICMR, New Delhi (2018-2021). PI: Dr. DV Singh, ILS, Bhubaneswar; PI: Dr. Saroj K. Mohapatra, NIBMG, Kalyani, West Bengal.
2. Studies on antibiotic resistance, virulence pattern and biofilm formation among Staphylococcus haemolyticus strains isolated from ocular infection. DST, New Delhi (2013-2016). PI: Dr. DV Singh, ILS, Bhubaneswar
3. Ocular infections caused by Staphylococcus species: Study of clinico-microbiologic features, virulence markers and genome profile. DST, New Delhi (2009-2012). PI: Dr. DV Singh, ILS, Bhubaneswar; PI: Dr. Savitri Sharma, LVEI, Bhubaneswar
4. Molecular analysis of environmental Vibrio cholerae strains: relationships with clinical isolates. DST, New Delhi (2007-2010). PI: Dr. DV Singh
5. Purification and characterization of a new toxin produced by candidate cholera vaccine strains. ICMR, New Delhi (2005-2008). PI: Dr. DV Singh; PCo-I: Dr. GP Chottray
6. Characterization of the new secretogenic toxin produced by Vibrio cholerae non-O1, non-O139 using panel of monoclonal antibodies. DST, Government of India, New Delhi (2000-2003). PI: Dr. DV Singh; PCo-I: Dr. GB Nair.
7. DST Young Scientist project: One (Not availed).

1. Elected Fellow: Biotech Research Society (India) (2004)
2. Member: Peer Review committee Department of Biotechnology, IIT, Guwahati (2014)
3. Member: Institutional Animal  Ethics Committee, OBPI, Bhubaneswar (2005-contd.)
4. Member: Doctoral Committee, NISER, Bhubaneswar (2015-contd.)
5. Member: Academic Council, Central University of South Bihar, Gaya
6. Member: Teachers Deputation Committee, CUSB, Gaya
7. Member: BoS Biotechnology, MG Central University, Motihari (2020-2022)
8. Chairman: BoS Biotechnology, CUSB, Gaya (2021-2023)
9. Cognate Member: BoS Life Science, CUSB, Gaya (2021-2023)
10. Cognate Member: BoS Environmental Science, CUSB, Gaya (2021-2023)
11. Cognate Member: BoS Bioinformatics, CUSB, Gaya (2021-2023)
12. Chairman: Institutional Animal Ethics Committee CUSB (2019-contd.)
13. Member: Institutional Ethics Committee CUSB (2020-contd.)
14. Member: Biosafety Committee CUSB (2020-contd.)
15. Associate Editor: Frontiers in Public Health (2021 onwards)
16. Review Editor: Frontiers in Public Health (2013-2021)
17. PhD Theis Examiner: KIIT, Bhubaneswar, BBA University, Lucknow, MG Kashi Vidyapith, Varanasi, Banaras Hindu University, Kerala University, Jadavpur University, Calcutta University, MS University Baroda, Dhaka University, Bangladesh.