Dr. Krishna Prakash

Transcription factors are protein that serves as molecular beacon for RNA polymerase to recognize the cis regulatory elements (e.g. a TATA box) of gene in order to regulate the gene expression. Around 10,000 Transcription factors are known from mammalian cells. Mutation and alteration in Transcription factors lead to cause diseases. For instance, Mutation in AP1 (Activated Protein) transcription factor gene causes the cancer in animals. In this regard, we are studying two mammalian transcription factors Interferon Regulatory Factor-1 & 2 (IRF-1 and IRF-2). Both Transcription factors belong to IRF family; biologically, control cell cycle regulation, hematopoiesis, immunomodulation and oncogenesis. IRF-1 is known to be tumor suppressor whereas IRF-2 is regarded as an oncogene. We are doing functional analysis of both the genes in vitro using recombinant DNA technology techniques. This study will pave way to decipher the mechanism of oncogenesis caused by both molecules also may help to devise drug to target these molecules to cure cancer.

• Publications:
1. Micronutrient and Redox Homeostasis Contribute to Moringa Oleifera-regulated Drought Tolerance in Wheat. (2021) Sahana Basu, Amlan A. Shashi Shekhar, Krishna Prakash, Ram Prasad, Gautam Kumar. Plant Growth Regulation. DOI: 10.21203/rs.3.rs-891395/v1
2. A rapid method for solubilization of chimeric human interferon regulatory factor-1 (IRF-1) protein in Escherichia coli Santosh Kumar Mishra 1, Ruchi Verma 1, Priyanka Kumari 1, Anjali Kumari 1, Krishna Prakash 2. Mol Biol Rep . 2021 Mar;48(3):2307-2314. doi: 10.1007/s11033-021-06260-w. Epub 2021 Mar 15.
3. Santosh KM, Nitish K, Gautam K, Tara K, Krishna P. Recombinant human interferon regulatory factor-1 (IRF-1) protein expression and solubilisation study in Escherichia coli. Mol Biol Rep. 2018 Oct;45(5):1367-1374. doi: 10.1007/s11033-018-4298-1. Epub 2018 Aug 16. PubMed PMID: 30117089. IF 2.017
4. Ghanshyam Kumar Satyapal, Santosh Kumar Mishra, Amrita Srivastava, Rajesh Kumar Ranjan, Krishna Prakash Rizwanul Haque, Nitish Kumar. Possible bioremediation of arsenic toxicity by isolating indigenous bacteria from the middle Gangetic plain of Bihar, India, Biotechnology Reports Volume 17, March 2018, Pages 117-125.
5. Santosh Kumar Mishra, Prabhat Suman, Pushpa Kumari Sharma, Nitish Kumar and Krishna Prakash* Human Interferon Regulatory Factor - 2 (Irf-2) : Chimeric Protein And Expression Analysis IJBPAS, September, 2015, 4(9): 5990 – 6002, ISSN:2277-4998
6. Gandhi, V.P., Priya, A. , Priya, S., Daiya, V. , Kesari, J., Prakash, K., Kumar Jha, Kumar, K. 4 , and Kumar, N. Isolation and molecular characterization of bacteria to heavy metals isolated from soil samples in Bokaro Coal Mines, India. Pollution,1(3): 287-295, Summer 2015 Print ISS2383-451X, IF
7. Prakash K, Kumar P, Mukherjee S, Rath PC. Chimeric murine interferon regulatory factor-2 (IRF-2) binds to IRF-E (IRF binding element), VREβ (virus response element) but not to VREα1. Cell Biochem Funct. 2014 Dec;32(8):630-6. doi: 10.1002/cbf.3050. Epub 2014 Sep 23. PubMed PMID: 25251598. IF: 2.142, ISSN: 1099-0844
8. Ajay Kumar, Krishna Prakash, Rajesh Kumar Sinha, Nitish Kumar. In Vitro Plant Propagation of Catharanthus roseus and Assessment of Genetic Fidelity of Micropropagated Plants by RAPD Marker Assay. Applied biochemistry and biotechnology . 20013. 01/2013; DOI: 10.1007/s12010-012-0010-4. 2013 Feb;169(3):894-900. doi: 10.1007/s12010-012-0010-4. Epub 2013 Jan 6. IF: 2.094, ISSN: 1559-0291
9. Gupta N, Chakrabarti K, Prakash K, Wadhwa N, Gupta T, Gupta SK. Immunogenicity and contraceptive efficacy of Escherichia coli-expressed recombinant porcine zona pellucida proteins. Am J Reprod Immunol. 2013 Aug;70(2):139-52. doi: 10.1111/aji.12095. Epub 2013 Feb 27. PubMed PMID: 23444974. Online ISSN:1600-0897 IF: 3.01
10. Krishna Prakash, Pramod C. Rath. Chimeric Oncogenic Interferon Regulatory Factor-2 (IRF-2): Degradation Products Are Biologically Active. A.J.M.B. Vol.2 No.4, October 2012. 371-379
11. Krishna Prakash and Pramod C. Rath. Replacement of the C-terminal tetrapeptide (314PAPV317 to 314SSSM317) in Interferon Regulatory Factor-2 alters its N-terminal DNA binding activity. J. Biosciences 35(4), December 2010. J Biosci. 2010 Dec;35(4):547-56. PubMed PMID: 21289437. ISSN: 0250-5991, IF: 1.823
12. Krishna Prakash and Pramod C. Rath. Mouse IRF-2: expression, Purification and the DNA binding activity. Molecular Biology Reports 2011 May 11. [Epub ahead of print] PubMed PMID: 21559834. 2012 Jan;39(1):599-606
13. Krishna Prakash and Renu Tuteja. A novel DEAD box helicase Has1p from Plasmodium falciparum: N-terminal is essential for activity. International parasitology. Parasitol Int. 2010 Jun; 59(2):271-7. Epub 2010 Feb 11 ISSN: 0020-7519, IF: 2.017
14. Satish K. Gupta, N. Gupta, P. Suman, S. Choudhurya, K. Prakash, T. Gupta, R. Sriraman, S. B. Nagendrakumar and V. A. Srinivasan. Contraceptive vaccines for human and animal utility. (2011) Journal of Reproductive Immunology Mar;88(2):240-6. Epub 2011 Mar. IF: 2.654
15. Pardeep Kumar1, Somnath Mukharjee1, Krishna Prakash1, R. K. Kale2, P. Mclean2 and Nazma zaheer baquer1. Antidiabetic effects of Trigonella foenum-graecum seed powder in a rat model. (2011). Toxicology and environmental chemistry J. Vol. 93, No. 10, December 2011, 2085–2097. IF 1.095, issn: 02772248
• Patent
Gupta SK, Gupta N, Chakrabarti K, Prakash K, Wadhwa N, Gupta T. Recombinant zona pellucida (ZP) proteins, vaccine composition and method of producing said vaccines. Indian Patent application # 1210/DEL/2013 (25/04/2013).

1. Fundamentals of Gene cloning, ISBN 9789388672610, Sara book publication, Ahmedabad, India (2020).
2. Expression and Mutational study of recombinant mouse IRF-2, ISBN-NR: 978-3-639-32929-2, VDM Publisher, Germany (2011).
3. Murine Interferon Regulatory Factor-2 (IRF-2) and Escherichia coli, 6139861012, Lambert academic book publishers (2018).

• Awards : BharatVikash award 2019, ISR, Bhubneswar, Odhisa, India.
• Projects : Start-up grant of UGC

1. Invited speaker - entitled “Truncated mutant Human IRF-1(1-233) shows the null effect on the expression of Bcl2, survivin, and Cyclin D in HEK 293T cell line: An anti-cancerous mutant protein ” at International conference on Innovative Research In Life Sciences for sustainable development organized by Botany Department of LNMU, Darbhanga Bihar (29th July to 31st July 2021).
2. oral presentation - entitled “Ammonium chloride, Lithium chloride and Mannitol enhance the solubilisation chimeric human Interferon Regulatory Factor-1 protein in E.coli.” at the International conference on Advances in Biosciences and Biotechnology-2021 (29thJanuary to 31st January) organised by Department of Biotechnology, Jaypee Institute of Information Technology, Noida, UP.
3. poster presented entitled “Expression analysis, optimization and solubilisation study of chimeric human Interferon Regulatory Factor-1 protein in E.coli.” at International conference on Advances in Biosciences and Biotechnology-2019 (31st January to 2nd February) organised by Department of Biotechnology, Jaypee Institute of Information Technology, Noida, UP.